Provide comprehensive cell sorting and phenotypic flow cytometry assay services
The Flow Cytometry Sorting Component of the Duke CFAR Immunology Core houses the only flow cytometry sorters at Duke capable of sorting live, unfixed human or animal derived cells, infected with HIV-1 or other pathogens requiring BSL2-enhanced or BSL3 containment and work practices. This capacity to sort live, unfixed HIV-1 infected cell subpopulations has broadened the scope of several ongoing, NIH-funded, AIDS research studies at Duke (e.g. CHAVI-ID).
The Analytical Flow Cytometry Component offers comprehensive flow cytometry assay services, including operator-assisted or investigator acquisition. The Analytical Flow Cytometry Component specializes in the development of highly standardized low- and high-dimensional polychromatic flow cytometry (PFC) assays for immune monitoring. Additional services include consultation (provided at no charge), assay development, custom polychromatic panel design, sample preparation, data analysis and archival, graphics and writing support (letters of intent, funding applications, publications, and presentations).