ABOUT THE WORKSHOP:
The flow-cytometry-based assays enable high through-put in vitro qualitative and quantitative analyses of phenotypic and functional markers expressed by antigen-specific B and T cellular subsets.
We have designed two sessions to meet different levels or interest by both basic science and clinical investigators. Importantly, we will provide information on how the investigators can leverage the combined expertise of the CFAR Immunology and DHVI Flow cytometry cores to support research of existing funded projects as well as new grant applications.
The first 90 minutes of the B & T-Cell Flow Cytometry Workshop will include sessions tailored for those new to flow cytometry who would like to learn the principles and basic applications of the assays but is also for those who would like a refresher. These sessions will cover the principles of the assay, including some basic technical aspects related to instruments and reagents, the importance of collection, preparation and handling of different sample types. Information about the cores’ expertise and accessibility will also be presented.
The remainder of the morning and the early part of the afternoon will provide more detailed and advanced information on how to investigate specific areas of antigen-specific B and T-cell responses. We will achieve this goal by hosting a breakout group for each cellular subset with a Q&A session.
Lastly, we will dedicate the balance of the afternoon to introduce the utilization of FlowJo software for data analysis.
Registration will be limited to the first 42 registrants.
Face coverings are currently optional per Duke’s current guidelines in the classroom setting, however this is subject to change and masking will be based on the guidelines as they stand on the day of the workshop.
Event Registration
Event Flyer - PDF
Event Agenda - PDF