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Analysis of Single-cell RNA sequencing of the B and T cell receptor antigen repertoire

Analysis of Single-cell RNA sequencing of the B and T cell receptor antigen repertoire

Recent technological advances now enable us to be able to simultaneously sequence the whole coding transcriptome, B cell and T cell receptor sequences and determine the antigen specificity of B cells and T cells from HIV infected or vaccinated individuals. Analyzing this large amount of data and developing tools to integrate these complex datasets to gain meaningful insights into HIV pathogenesis of vaccine biology remain a significant challenge. During this project, the intern would utilize the large databases of over 100,000 single-cell RNA sequencing data that our laboratory has generated to begin to setup a software pipeline that integrates these B or T cell receptor clonotype tracking with transcriptome analysis and develop new ways of visualizing this complex data. At the completion of the project the intern would have gained new knowledge of single-cell RNA sequencing technology, biology of HIV and B/T cell immunology, began to fill a critical void that is needed in most “omics” and that is integration with other “omics” data to generate the best possible mechanistic insights