Core Support Facilities

Flow Cytometry Core

Kent J. Weinhold, Director

John F. Whitesides, Co-Director

Janet S. Ottinger, Core Manager

Provides state-of-the-art, multi-color, BSL-3 flow cytometric analytic and fluorescence activated cell sorting support for basic, translational, and clinical investigations conducted by Duke CFAR members, including sorting of HIV-1 infected cells.
Seeks to enhance the scientific approaches used by Duke CFAR Investigators by educating the Core User base in more effective ways of utilizing the state-of-the-art tools offered by the Flow Cytometry Core Facility, as well as to provide programmatic advancement for Managers and Operators of the Facility.

The Flow Cytometry Core Facility is comprised of two Component Labarotories, both housed in a BSL-3 biocontainment laboratory within the SORF Building. The labs are closely linked physically, administratively, and scientifically, in order to form a highly interactive and unified Flow Cytometry Facility. The BSL-3 Analytical Component Laboratory provides a broad range of polychromatic flow cytometry applications to CFAR investigators and, together with the BSL-3 Sorting Component Laboratory, comprises the only flow cytometry research facility on campus that routinely processes, sorts, and analyzes HIV-1 infected specimens. The BSL-3 Sorting Lab's flow cytometer is capable of sorting live, unfixed human or animal-derived cells, infected with HIV-1 or equivalent pathogens.